Anti-Dystrophin antibody
| Name | Anti-Dystrophin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab85302 |
| Prices | $376.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB |
| Species Reactivities | Human, Mouse, Rat, Chicken, Bovine, Dog, Pig, Monkey, Hamster, Orangutan |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1850 - 1950 of Human Dystrophin |
| Description | Rabbit Polyclonal |
| Gene | DMD |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of Dystrophin staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85302, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Anti-Dystrophin antibody (ab85302) at 1 µg/ml + Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330) at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.