ab15278 staining Dystrophin in transverse sections of tibialis anterior (TA) muscles of mdx mice by Immunohistochemistry (Frozen sections). Mice were killed and TA muscles were removed, pinned to parafilm-covered cork, snap frozen in liquid N2-cooled isopentane, and stored at -80°C. Sections were cut using cryostat and blocked with 10% normal goat serum in 1% BSA/PBS for 1 h and then incubated with primary antibody for 1 hour. A Cy3 conjugated anti rabbit IgG at 1/500 dilution was used as secondary. Slides were coverslipped with Vectashield mounting medium with DAPI and imaged.
Immunohistochemical staining of human skeletal muscle with ab15278