Anti-EIF2S1 (phospho S51) antibody [E90]
| Name | Anti-EIF2S1 (phospho S51) antibody [E90] |
|---|---|
| Supplier | Abcam |
| Catalog | ab32157 |
| Prices | $404.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | E90 |
| Applications | ICC/IF ICC/IF WB IHC-P FC DB IHC-F |
| Species Reactivities | Mouse, Rat, Human, Drosophila, Plant, Monkey, Neurospora crassa, Bovine, Pig |
| Antigen | Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human EIF2S1 (phospho S51) |
| Blocking Peptide | EIF2S1 (phospho S51) peptide |
| Description | Rabbit Monoclonal |
| Gene | eIF-2alpha |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Overlay histogram showing HeLa cells stained with ab32157 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32157, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) at 1/500 dilution + PC12 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/12600_ab32157wb.gif)
Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) at 1/500 dilution + PC12 cell lysate
Immunohistochemical analysis of paraffin-embedded human liver carcinoma using ab32157 at 1/50 dilution.Immunohistochemical analysis of paraffin-embedded human liver carcinoma using ab32157 at 1/50 dilution.
Dot blot analysis on antigen peptide. A nitrocellulose membrane was spotted with (1) phospho-peptide and (2) non-phospho-peptide at 5, 1, and 0.1 ng, and then blotted with ab32157 at 1:500 dilution.
ab32157 at 1/100 staining human epithelial cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA before incubation with the antibody for 1 hour. An Alexa-Fluor ® 647 conjugated goat anti-rabbit IgG was used as the secondary.See Abreview
ab32157 showing positive staining in Breast carcinoma tissue.
ab32157 showing positive staining in Cervical carcinoma tissue.
ab32157 showing positive staining in Colonic adenocarcinoma tissue.
ab32157 showing positive staining in Hepatocellular carcinoma tissue.
Product References
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