Anti-eIF4A1 antibody [EPR14506]

• Supplier: Abcam
• Catalog: ab185946
• Prices: $376.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR14506
• Applications: WB IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human eIF4A1 aa 300-400
• Description: Rabbit Monoclonal
• Gene: EIF4A1
• Conjugate: Unconjugated

Product images

All lanes : Anti-eIF4A1 antibody [EPR14506] (ab185946) at 1/10000 dilutionLane 1 : Jurkat cell lysateLane 2 : 293 cell lysateLane 3 : HeLa cell lysateLane 4 : MCF7 cell lysateLysates/proteins at 20 µg per lane.Secondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

All lanes : Anti-eIF4A1 antibody [EPR14506] (ab185946) at 1/1000 dilutionLane 1 : rat C6 cell lysateLane 2 : mouse Raw264.7 cell lysateLane 3 : rat PC12 cell lysateLane 4 : mouse NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Western blot analysis on immunoprecipitation from 1) 293 cell lysate and 2) PBS, labeling eIF4A1 using ab185946 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.