Anti-eIF4G1 antibody
| Name | Anti-eIF4G1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab47649 |
| Prices | $382.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Human, Mouse |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human eIF4G1 |
| Description | Rabbit Polyclonal |
| Gene | EIF4G1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-eIF4G1 antibody (ab47649) at 1 µg/ml + HEK293 Human embryonic kidney cell line Whole Cell Lysate at 10 µgSecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
ICC/IF image of ab47649 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab47649, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Product References
5-Fluorouracil affects assembly of stress granules based on RNA incorporation. - 5-Fluorouracil affects assembly of stress granules based on RNA incorporation.
Kaehler C, Isensee J, Hucho T, Lehrach H, Krobitsch S. Nucleic Acids Res. 2014 Jun;42(10):6436-47.
Ataxin-2-like is a regulator of stress granules and processing bodies. - Ataxin-2-like is a regulator of stress granules and processing bodies.
Kaehler C, Isensee J, Nonhoff U, Terrey M, Hucho T, Lehrach H, Krobitsch S. PLoS One. 2012;7(11):e50134.
Nonproteasomal targets of the proteasome inhibitors bortezomib and carfilzomib: a - Nonproteasomal targets of the proteasome inhibitors bortezomib and carfilzomib: a
Arastu-Kapur S, Anderl JL, Kraus M, Parlati F, Shenk KD, Lee SJ, Muchamuel T, Bennett MK, Driessen C, Ball AJ, Kirk CJ. Clin Cancer Res. 2011 May 1;17(9):2734-43.