Anti-eIF5A2 antibody [EPR7411]

• Supplier: Abcam
• Catalog: ab126735
• Prices: $313.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR7411
• Applications: WB IP IHC-P FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide, corresponding to amino acid residues in Human eIF5A2
• Description: Rabbit Monoclonal
• Gene: EIF5A2
• Conjugate: Unconjugated

Product images

Overlay histogram showing HeLa cells stained with ab126735 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126735, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-eIF5A2 antibody [EPR7411] (ab126735) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : HeLa cell lysateLane 3 : A431 cell lysateLane 4 : CaCo 2 cell lysateLane 5 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat-anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique

ab126735 at 1/100 dilution, staining eIF5A2 in Formalin fixed Paraffin-embedded Human ovarian carcinoma tissue by Immunohistochemistry.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD