Anti-Elafin/Skalp antibody
| Name | Anti-Elafin/Skalp antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab46774 |
| Prices | $376.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Synthetic peptide based on the middle of human Elafin/Skalp |
| Blocking Peptide | Elafin/Skalp peptide |
| Description | Rabbit Polyclonal |
| Gene | PI3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Elafin/Skalp antibody (ab46774) at 1/1000 dilutionLane 1 : recombinant Human Elafin at 0.05 µgLane 2 : recombinant Human Elafin at 0.01 µgLane 3 : recombinant Human Elafin at 0.001 µg
ICC/IF image of ab46774 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab46774, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.