All lanes : Anti-Ephrin B3 antibody (ab53063) at 1/500 dilutionLane 1 : Extracts from SKOV3 cellsLane 2 : Extracts from SKOV3 cells treated with peptide
Immunohistochemical analysis of paraffin-embedded human brain tissue using ab53063 at a 1:50 dilution.Left image un-treated.Right image treated with peptide.
ICC/IF image of ab53063 stained SKNSH cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53063, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.