All lanes : Anti-Factor X antibody [EPR16249] (ab196023) at 1/20000 dilutionLane 1 : Human fetal liver lysateLane 2 : Human blood lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-Factor X antibody [EPR16249] (ab196023) at 1/20000 dilution + Human plasma lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Anti-Factor X antibody [EPR16249] (ab196023) at 1/20000 dilution + HepG2 (Human liver hepatocellular carcinoma) cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Factor X with ab196023 at 1/1200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human liver tissue is observed. Counter stained with Hematoxylin.Secondary control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.