Anti-FANCA antibody [EPR16519]

• Supplier: Abcam
• Catalog: ab201457
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16519
• Applications: IP WB
• Species Reactivities: Human
• Antigen: Recombinant fragment within Human FANCA aa 1-150
• Description: Rabbit Monoclonal
• Gene: FANCA
• Conjugate: Unconjugated

Product images

All lanes : Anti-FANCA antibody [EPR16519] (ab201457) at 1/10000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLane 2 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysateLane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysateLane 4 : A431 (Human epidermoid carcinoma) whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-FANCA antibody [EPR16519] (ab201457) at 1/1000 dilution + Human colon lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

FANCA was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab201457 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab201457 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/1500 dilution.Lane 1: HeLa whole cell lysate 10 µg (Input). Lane 2: ab201457 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab201457 in HeLa whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 30 seconds.