All lanes : Anti-FANCB antibody (ab86136) at 0.1 µg/mlLane 1 : Whole cell lysate from HeLa cells at 50 µgLane 2 : Whole cell lysate from HeLa cells at 15 µgLane 3 : Whole cell lysate from HeLa cells at 5 µgLane 4 : Whole cell lysate from 293T cells at 50 µg
Immunoprecipitation/ Western Blot of ab86136 Lane 1: ab86136 at 10µg/mg whole cell lysate. Lane 2: Control IgG. ab86136 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Chemiluminescence with an exposure time of 10 seconds.
ab86136 staining FANCB in HeLa cells by Immunocytochemistry/ Immunoflourescence. Cells were PFA-fixed and permeabilized in 2% Triton X-100 prior to blocking in 7% Serum for 1 hour at room temperature. The primary antibody was diluted 1/100 and incubated with the sample for 17 hours at 4°C. The secondary antibody was an Alexa Fluor® 555-conjugated Donkey anti-Rabbit polyclonal, used undiluted.See Abreview