Anti-FBP1 antibody [EPR4620]
| Name | Anti-FBP1 antibody [EPR4620] |
|---|---|
| Supplier | Abcam |
| Catalog | ab109732 |
| Prices | $357.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPR4620 |
| Applications | ICC/IF ICC/IF WB IP ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to residues in Human FBP1 |
| Description | Rabbit Monoclonal |
| Gene | FBP1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab109732 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109732, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![All lanes : Anti-FBP1 antibody [EPR4620] (ab109732) at 1/1000 dilutionLane 1 : Human stomach lysateLane 2 : Human fetal liver lysateLane 3 : MCF7 lysateLysates/proteins at 10 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/17850_FBP1-Primary-antibodies-ab109732-1.jpg)
All lanes : Anti-FBP1 antibody [EPR4620] (ab109732) at 1/1000 dilutionLane 1 : Human stomach lysateLane 2 : Human fetal liver lysateLane 3 : MCF7 lysateLysates/proteins at 10 µg per lane.