Anti-FGFR1 alpha antibody [M2F12]
| Name | Anti-FGFR1 alpha antibody [M2F12] |
|---|---|
| Supplier | Abcam |
| Catalog | ab829 |
| Prices | $384.00 |
| Sizes | 125 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | M2F12 |
| Applications | ICC/IF ICC/IF IHC-P IHC-F IP WB |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Recombinant human ectodomain of FGFr1a expressed in E |
| Description | Mouse Monoclonal |
| Gene | FGFR1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab829 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab829, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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Fibroblast growth factor receptor gene expression and immunoreactivity are - Fibroblast growth factor receptor gene expression and immunoreactivity are
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Modification of alternative messenger RNA splicing of fibroblast growth factor - Modification of alternative messenger RNA splicing of fibroblast growth factor
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