Anti-FOXA1 antibody - ChIP Grade
| Name | Anti-FOXA1 antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab23738 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F WB IHC-P ChIP ChIP ChIP EMSA ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human, Rat, Xenopus |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human FOXA1 |
| Description | Rabbit Polyclonal |
| Gene | FOXA1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-FOXA1 antibody - ChIP Grade (ab23738) at 1 µg/ml + Human heart tissue lysate at 20 µgSecondaryGoat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
(Image courtesy of Human Protein Atlas) ab23738 staining FOXA1 in Human prostate. Paraffin embedded human prostate tissue was incubated with ab23738 (1/200 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab23738 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
All lanes : Anti-FOXA1 antibody - ChIP Grade (ab23738) at 1/1000 dilutionLane 1 : Lysate prepared from human LNCaP cellsLane 2 : Lysate prepared from MCF7 cellsLane 3 : Lysate prepared from human LNCaP cells with ethanol as vehicle controlLane 4 : Lysate prepared from human LNCaP cells with 100 nM DHTLysates/proteins at 20 µg per lane.SecondaryHRP-conjugated goat polyclonal to rabbit IgG at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab23738 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab23738 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
FOXA1 promotes tumor cell proliferation through AR involving the Notch pathway in - FOXA1 promotes tumor cell proliferation through AR involving the Notch pathway in
Qiu M, Bao W, Wang J, Yang T, He X, Liao Y, Wan X. BMC Cancer. 2014 Feb 11;14:78.
Switch in FOXA1 status associates with endometrial cancer progression. - Switch in FOXA1 status associates with endometrial cancer progression.
Tangen IL, Krakstad C, Halle MK, Werner HM, Oyan AM, Kusonmano K, Petersen K, Kalland KH, Akslen LA, Trovik J, Hurtado A, Salvesen HB. PLoS One. 2014 May 21;9(5):e98069.
Cooperativity and equilibrium with FOXA1 define the androgen receptor - Cooperativity and equilibrium with FOXA1 define the androgen receptor
Jin HJ, Zhao JC, Wu L, Kim J, Yu J. Nat Commun. 2014 May 30;5:3972.
SUMOylation modulates the transcriptional activity of androgen receptor in a - SUMOylation modulates the transcriptional activity of androgen receptor in a
Sutinen P, Malinen M, Heikkinen S, Palvimo JJ. Nucleic Acids Res. 2014 Jul;42(13):8310-9.
Dynamic estrogen receptor interactomes control estrogen-responsive trefoil Factor - Dynamic estrogen receptor interactomes control estrogen-responsive trefoil Factor
Quintin J, Le Peron C, Palierne G, Bizot M, Cunha S, Serandour AA, Avner S, Henry C, Percevault F, Belaud-Rotureau MA, Huet S, Watrin E, Eeckhoute J, Legagneux V, Salbert G, Metivier R. Mol Cell Biol. 2014 Jul;34(13):2418-36.
Genetic variants within the second intron of the KCNQ1 gene affect CTCF binding - Genetic variants within the second intron of the KCNQ1 gene affect CTCF binding
Demars J, Shmela ME, Khan AW, Lee KS, Azzi S, Dehais P, Netchine I, Rossignol S, Le Bouc Y, El-Osta A, Gicquel C. J Med Genet. 2014 Aug;51(8):502-11.
Identification of a regulatory variant that binds FOXA1 and FOXA2 at the - Identification of a regulatory variant that binds FOXA1 and FOXA2 at the
Fogarty MP, Cannon ME, Vadlamudi S, Gaulton KJ, Mohlke KL. PLoS Genet. 2014 Sep 11;10(9):e1004633.
FoxA1 specifies unique androgen and glucocorticoid receptor binding events in - FoxA1 specifies unique androgen and glucocorticoid receptor binding events in
Sahu B, Laakso M, Pihlajamaa P, Ovaska K, Sinielnikov I, Hautaniemi S, Janne OA. Cancer Res. 2013 Mar 1;73(5):1570-80.
Androgen receptor-independent function of FoxA1 in prostate cancer metastasis. - Androgen receptor-independent function of FoxA1 in prostate cancer metastasis.
Jin HJ, Zhao JC, Ogden I, Bergan RC, Yu J. Cancer Res. 2013 Jun 15;73(12):3725-36.
KDM4B is a master regulator of the estrogen receptor signalling cascade. - KDM4B is a master regulator of the estrogen receptor signalling cascade.
Gaughan L, Stockley J, Coffey K, O'Neill D, Jones DL, Wade M, Wright J, Moore M, Tse S, Rogerson L, Robson CN. Nucleic Acids Res. 2013 Aug;41(14):6892-904.