Anti-G-CSF antibody [EPR3203(N)(B)]

• Supplier: Abcam
• Catalog: ab181053
• Prices: $384.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR3203(N)(B)
• Applications: WB ICC/IF ICC/IF IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MCSF aa 150 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: CSF3
• Conjugate: Unconjugated

Product images

Immunofluorescent analysis of HT-1376 cells (paraformaldehyde-fixed, 4%) labeling G-CSF with ab181053 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

All lanes : Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053) at 1/2000 dilutionLane 1 : K562 cell lysateLane 2 : KM3 cell lysateLane 3 : NCI-H460 cell lysateLane 4 : HT-1376 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution

Western blot analysis of immunoprecipitation pellet from K562 cell lysate (lane 1) or a Negative control (lane 2)  immunoprecipitated using ab181053 at 1/20 dilution.Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.