Anti-gamma Catenin antibody [15F11]
| Name | Anti-gamma Catenin antibody [15F11] |
|---|---|
| Supplier | Abcam |
| Catalog | ab12083 |
| Prices | $379.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 15F11 |
| Applications | ICC/IF ICC/IF FC WB IP |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | This information is considered to be commercially sensitive |
| Description | Mouse Monoclonal |
| Gene | JUP |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab12083 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12083, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.
![Overlay histogram showing HeLa cells stained with ab12083 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12083, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/22488_gamma-Catenin-Primary-antibodies-ab12083-2.jpg)
Overlay histogram showing HeLa cells stained with ab12083 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12083, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Identification of plakoglobin domains required for association with N-cadherin - Identification of plakoglobin domains required for association with N-cadherin
Sacco PA, McGranahan TM, Wheelock MJ, Johnson KR. J Biol Chem. 1995 Aug 25;270(34):20201-6.
P- and E-cadherin are in separate complexes in cells expressing both cadherins. - P- and E-cadherin are in separate complexes in cells expressing both cadherins.
Johnson KR, Lewis JE, Li D, Wahl J, Soler AP, Knudsen KA, Wheelock MJ. Exp Cell Res. 1993 Aug;207(2):252-60.