![Overlay histogram showing MCF7 cells stained with ab119908 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119908, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/22489_ab119908-7-ab119908FC.jpg)
Overlay histogram showing MCF7 cells stained with ab119908 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119908, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![Anti-gamma Catenin antibody [4C12] (ab119908) at 1/500 dilution + Recombinant Human gamma Catenin protein](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/22490_gamma-Catenin-Primary-antibodies-ab119908-1.JPG)
Anti-gamma Catenin antibody [4C12] (ab119908) at 1/500 dilution + Recombinant Human gamma Catenin protein
![All lanes : Anti-gamma Catenin antibody [4C12] (ab119908) at 1/500 dilutionLane 1 : T47D cell lysateLane 2 : MCF7 cell lysateLane 3 : SKBR 3 cell lysateLane 4 : A431 cell lysateLane 5 : HEK293 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/22491_gamma-Catenin-Primary-antibodies-ab119908-2.JPG)
All lanes : Anti-gamma Catenin antibody [4C12] (ab119908) at 1/500 dilutionLane 1 : T47D cell lysateLane 2 : MCF7 cell lysateLane 3 : SKBR 3 cell lysateLane 4 : A431 cell lysateLane 5 : HEK293 cell lysate
ab119908 at 1/200 dilution staining gamma Catenin in paraffin-embedded Human rectum tissues by Immunohistochemistry followed by DAB staining.
ab119908 at 1/200 dilution staining gamma Catenin in paraffin-embedded Human stomach cancer tissues by Immunohistochemistry followed by DAB staining.
ELISA image showing use of ab119908.
ab119908 at 1/200 dilution staining gamma Catenin in U251 cells by Immunofluorescence (green). Blue: DRAQ5 fluorescent DNA dye.