Anti-gamma H2A.X (phospho S140) antibody [3F2]
| Name | Anti-gamma H2A.X (phospho S140) antibody [3F2] |
|---|---|
| Supplier | Abcam |
| Catalog | ab22551 |
| Prices | $403.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 3F2 |
| Applications | WB ICC/IF ICC/IF IHC-P ELISA FC |
| Species Reactivities | Mouse, Rat, Bovine, Human |
| Antigen | Other Immunogen Type corresponding to Human gamma H2A |
| Description | Mouse Monoclonal |
| Gene | H2AFX |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab22551 labelling gamma H2A.X (phospho S140) in HeLa cells by immunocytochemistry/immunofluorescence.
ab22551 labelling gamma H2A.X (phospho S140) in control (left) and G2/M Arrested (right) cells by Immunocytochemistry/Immunofluorescence.
Western blot analysis of Phospho-H2A.X pSer140(ab22551) at a concentration of 1 ug/ml on Jurkat cell untreated (Lane 1) and Jurkat cell stimulated with staurosporine (Lane 2) followed by HRP conjugated goat anti-mouse lgG (H+L) Secondary Antibody.
![All lanes : Anti-gamma H2A.X (phospho S140) antibody [3F2] (ab22551)Lane 1 : gamma irradiated HeLa cells: Unphosphorylated H2AX control Lane 2 : gamma irradiated HeLa cells: Phosphorylated H2AX](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/22546_ab22551_1.JPG)
All lanes : Anti-gamma H2A.X (phospho S140) antibody [3F2] (ab22551)Lane 1 : gamma irradiated HeLa cells: Unphosphorylated H2AX control Lane 2 : gamma irradiated HeLa cells: Phosphorylated H2AX
Immunofluorescence detection of phosphorylated H2AX in gamma irradiated HeLa cells using ab22551.
IHC image of ab22551 staining in Human breast formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22551, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ICC/IF image of ab22551 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22551, 10µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Overlay histogram showing HeLA cells stained with ab22551 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22551, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/22550_gamma-H2AX-Primary-antibodies-ab22551-3.jpg)
Overlay histogram showing HeLA cells stained with ab22551 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22551, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
![A431 cell nucleus (DAPI signal) showing three gamma-H2AX foci structures. Primary anitibody H2A.X (phospho S140) antibody [3F2], ab22551, 100ug.Secondary antibody Mouse IgG-Fc (FITC), ab97264, 1mg.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/22551_gamma-H2AX-Primary-antibodies-ab22551-8.jpg)
A431 cell nucleus (DAPI signal) showing three gamma-H2AX foci structures. Primary anitibody H2A.X (phospho S140) antibody [3F2], ab22551, 100ug.Secondary antibody Mouse IgG-Fc (FITC), ab97264, 1mg.
Product References
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