Anti-GAPDH antibody
| Name | Anti-GAPDH antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab70699 |
| Prices | $385.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P WB |
| Species Reactivities | Mouse, Human, Rat, Chicken, Guinea Pig, Turkey, Pig, Xenopus, Chimpanzee, Monkey, Ape |
| Antigen | Synthetic peptide, corresponding to a region between residues 250-300 of Human GAPDH |
| Description | Rabbit Polyclonal |
| Gene | GAPDH |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling GAPDH with ab70699 at 1/200 (1µg/ml). Detection: DAB.
ab70699 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab70699 at 5µg/ml overnight at +4°C. The secondary antibody (green) was anti-Rabbit DyLight® 488 (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-GAPDH antibody (ab70699) at 0.04 µg/mlLane 1 : Whole cell lysate from HeLa cells at 50 µgLane 2 : Whole cell lysate from HeLa cells at 15 µgLane 3 : Whole cell lysate from HeLa cells at 5 µgLane 4 : Whole cell lysate from NIH 3T3 cells at 50 µg
developed using the ECL techniquePerformed under reducing conditions.
developed using the ECL techniquePerformed under reducing conditions.
IHC image of GAPDH staining in Human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70699, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
Prenylome profiling reveals S-farnesylation is crucial for membrane targeting and - Prenylome profiling reveals S-farnesylation is crucial for membrane targeting and
Charron G, Li MM, MacDonald MR, Hang HC. Proc Natl Acad Sci U S A. 2013 Jul 2;110(27):11085-90. doi:
S-palmitoylation and ubiquitination differentially regulate interferon-induced - S-palmitoylation and ubiquitination differentially regulate interferon-induced
Yount JS, Karssemeijer RA, Hang HC. J Biol Chem. 2012 Jun 1;287(23):19631-41.