Anti-GATA3 antibody [EPR16651]

• Supplier: Abcam
• Catalog: ab199428
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16651
• Applications: WB IHC-P ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant fragment within Human GATA3 aa 1-200
• Description: Rabbit Monoclonal
• Gene: GATA3
• Conjugate: Unconjugated

Product images

Anti-GATA3 antibody [EPR16651] (ab199428) at 1/1000 dilution + SH-SY5Y (Human neuroblastoma from bone marrow cells) cell extract at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-GATA3 antibody [EPR16651] (ab199428) at 1/1000 dilutionLane 1 : C6 (rat glial tumor cells) cell extractLane 2 : RAW 264.7 (mouse macrophage cells transformed with Abelson murine leukemia) cell extractLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human neuroblastoma tissue labeling GATA3 with ab199428 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Human neuroblastoma tissue is observed. Counter stained with Hematoxylin.Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling GATA3 with ab199428 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Human breast carcinoma tissue is observed. Counter stained with Hematoxylin.Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y cells (Human neuroblastoma from bone marrow cells) labeling GATA3 with ab199428 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on SH-SY5Y cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:--ve control 1 - ab199428 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

GATA3 was immunoprecipitated from 1mg of RAW 264.7 (mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell extract with ab199428 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199428 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: RAW 264.7 whole cell extract. Lane 2: RAW 264.7 whole cell extract following immunoprecipitation. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199428 in RAW 264.7 whole cell extract.Blocking and dilution buffer and concentration: 5% NFDM/TBST.