Anti-Glucose 6 Phosphate Dehydrogenase antibody

• Supplier: Abcam
• Catalog: ab993
• Prices: $398.00
• Sizes: 50 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-F IP WB ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human, Chimpanzee, Monkey, Monkey, Ape
• Antigen: Synthetic peptide (Human) conjugated to KLH - which represented a portion of human Glucose-6-Phosphate Dehydrogenase encoded in part by exon 6 and in part by exon 7 (LocusLink ID 2539)
• Blocking Peptide: Human Glucose 6 Phosphate Dehydrogenase peptide
• Description: Rabbit Polyclonal
• Gene: G6PD
• Conjugate: Unconjugated

Product images

ICC/IF image of ab993 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab993 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ab993 staining Glucose 6 phosphate dehydrogenase in Mouse skeletal muscle tissue sections by IHC-Fr (frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 2 hours at 25°C. Samples were incubated with primary antibody (1/500 in PBS Tween 20) for 12 hours at 4°C. An Alexa Fluor®-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as secondary antibody.See Abreview

All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993) at 0.6 µg/mlLane 1 : HUVEC whole cell lysate at 15 µgLane 2 : HUVEC whole cell lysate at 25 µgdeveloped using the ECL technique

Western Blot Lanes 1-2 : Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993) at 0.5 µg/mlImmunoprecipitation Lanes 3-4 : Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993) at 3.75 µg/mg lysate. Lane 1 : Wild-type mouse peritoneal macrophage whole cell lysate at 20 µg Lane 2 : Glucose 6 Phosphate Dehydrogenase deficient mouse peritoneal macrophage whole cell lysate at 20 µgLane 3 : Supernatant from wild-type mouse peritoneal macrophage whole cell lysate at 1 mgLane 4 : Immunoprecipitate from wild-type mouse peritoneal macrophage whole cell lysate at 1 mg

Product References

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