Anti-Glutathione Peroxidase 1 antibody [EPR3312]
| Name | Anti-Glutathione Peroxidase 1 antibody [EPR3312] |
|---|---|
| Supplier | Abcam |
| Catalog | ab108427 |
| Prices | $397.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPR3312 |
| Applications | WB IP IHC-P FC ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human, Marmoset |
| Antigen | Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence) |
| Blocking Peptide | Glutathione Peroxidase 1 peptide |
| Description | Rabbit Monoclonal |
| Gene | GPX1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Overlay histogram showing HepG2 cells stained with ab108427 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108427, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![All lanes : Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427) at 1/1000 dilutionLane 1 : Human fetal liver lysateLane 2 : 293T cell lysateLane 3 : THP1 cell lysateLane 4 : HepG2 cell lysateLysates/proteins at 10 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/25537_Glutathione-Peroxidase-1-Primary-antibodies-ab108427-6.jpg)
All lanes : Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427) at 1/1000 dilutionLane 1 : Human fetal liver lysateLane 2 : 293T cell lysateLane 3 : THP1 cell lysateLane 4 : HepG2 cell lysateLysates/proteins at 10 µg per lane.
ab108427, at 1/100 dilution, staining Glutathione Peroxidase 1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
ab108427, at 1/100 dilution, staining Glutathione Peroxidase 1 in 293T cells by Immunofluorescence.