Anti-Glycogen synthase 1 antibody [EP816Y]
| Name | Anti-Glycogen synthase 1 antibody [EP816Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab40867 |
| Prices | $385.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP816Y |
| Applications | IHC-F WB IP ICC/IF IHC-P |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to residues near the N-terminus of the human glycogen synthase |
| Description | Rabbit Monoclonal |
| Gene | GYS1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-Glycogen synthase 1 antibody [EP816Y] (ab40867) at 1/20000 dilution + HeLa cell lysate at 10 µg](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/25784_ab40867wb.jpg)
Anti-Glycogen synthase 1 antibody [EP816Y] (ab40867) at 1/20000 dilution + HeLa cell lysate at 10 µg
Immunohistochemical staining of paraffin-embedded human muscle using anti-Glycogen Synthase 1 ab40867 diluted 1:250.
ICC/IF image of ab40867 stained A431 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40867, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.