Anti-GRK1 antibody [D11]

• Supplier: Abcam
• Catalog: ab2776
• Prices: $370.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D11
• Applications: ICC/IF ICC/IF IHC-F WB IP
• Species Reactivities: Mouse, Bovine, Human
• Antigen: Full length native protein (purified) corresponding to Human GRK1
• Description: Mouse Monoclonal
• Gene: GRK1
• Conjugate: Unconjugated

Product images

Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in murine cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2776 (1:200) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2776 (1:200) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in human cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2776 (1:20) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

Immunolocalization of GRK1 in bovine retina using ab2776.

Product References

Variation in rhodopsin kinase expression alters the dim flash response shut off - Variation in rhodopsin kinase expression alters the dim flash response shut off

Sakurai K, Young JE, Kefalov VJ, Khani SC. Invest Ophthalmol Vis Sci. 2011 Aug 29;52(9):6793-800.

Recoverin binds exclusively to an amphipathic peptide at the N terminus of - Recoverin binds exclusively to an amphipathic peptide at the N terminus of

Higgins MK, Oprian DD, Schertler GF. J Biol Chem. 2006 Jul 14;281(28):19426-32. Epub 2006 May 4.