![Overlay histogram showing HeLa cells stained with ab113643 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab113643, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/27689_ab113643-5-ab113643FC.jpg)
Overlay histogram showing HeLa cells stained with ab113643 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab113643, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![All lanes : Anti-GRK2 antibody [3F8] (ab113643) at 1/500 dilutionLane 1 : HeLa cell lysateLane 2 : Jurkat cell lysateLane 3 : MOLT4 cell lysateLane 4 : Raji cell lysateLane 5 : THP-1 cell lysateLane 6 : L1210 cell lysateLane 7 : Cos7 cell lysateLane 8 : Pc-12 cell lysateLane 9 : NIH3T3 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/27690_GRK2-Primary-antibodies-ab113643-1.jpg)
All lanes : Anti-GRK2 antibody [3F8] (ab113643) at 1/500 dilutionLane 1 : HeLa cell lysateLane 2 : Jurkat cell lysateLane 3 : MOLT4 cell lysateLane 4 : Raji cell lysateLane 5 : THP-1 cell lysateLane 6 : L1210 cell lysateLane 7 : Cos7 cell lysateLane 8 : Pc-12 cell lysateLane 9 : NIH3T3 cell lysate
ab113643 at 1/100000 detecting various concentrations of antigen.
Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue using ab113643 at a dilution of 1/200.
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue using ab113643 at a dilution of 1/200.