Western blot analysis on immunoprecipitation from 1) K562 cell lysate and 2) PBS, labeling HB EGF using ab185555 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.
All lanes : Anti-HB EGF antibody [EPR13276(2)] (ab185555) at 1/20000 dilutionLane 1 : HeLa cell lysateLane 2 : K562 cell lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique