HEK293T cells transfected with either RC201745 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab114023 at 1/100, and then analyzed by flow cytometry.
Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HDAC1, using ab114023 at 1/100.
All lanes : Anti-HDAC1 antibody [5C3] (ab114023) at 1/200 dilutionLane 1 : HEK293T cells were transfected with the pCMV6-ENTRY control cDNA for 48 hrs and lysed Lane 2 : HEK293T cells were transfected with the pCMV6-ENTRY HDAC1 cDNA for 48 hrs and lysed Lysates/proteins at 5 µg per lane.
All lanes : Anti-HDAC1 antibody [5C3] (ab114023) at 1/200 dilutionLane 1 : HepG2 cell lysateLane 2 : HeLa cell lysateLane 3 : HT29 cell lysateLane 4 : A549 cell lysateLane 5 : COS7 cell lysateLane 6 : Jurkat cell lysateLane 7 : MDCK cell lysateLane 8 : PC12 cell lysateLane 9 : MCF7 cell lysateLysates/proteins at 35 µg per lane.