Anti-HDAC1 antibody - ChIP Grade

• Supplier: Abcam
• Catalog: ab7028
• Prices: $403.00
• Sizes: 50 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB IP IHC-P ChIP ChIP ChIP ICC/IF ICC/IF IHC-F
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide: C-KEEKPEAKGVKEEVKLA conjugated to KLH, corresponding to amino acids 466-482 of Human HDAC 1
• Description: Rabbit Polyclonal
• Gene: HDAC1
• Conjugate: Unconjugated

Product images

HeLa cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone. Fixed cells were stained with 1:200 ab7028. The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.

 Sonicated Chromatin prepared from untreated (UI) or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure with ab7028 to HDAC1 and the immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are nomalized over inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 5 µl of ab7028 and 2x10.

All lanes : Anti-HDAC1 antibody - ChIP Grade (ab7028) at 1/1000 dilutionLane 1 : Whole cell lysate from human HEK293 cell line Lane 2 : Whole cell lysate from human HEK293 cell line treated with HDAC1 gene silencing shRNALysates/proteins at 20 µg per lane.SecondaryHRP-conjugated goat anti-rabbit Ig at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-HDAC1 antibody - ChIP Grade (ab7028) at 1/2000 dilution + Human HuH-7 whole cell lysate at 15 µgSecondaryHRP conjugated Sheet anti-rabbit IgG polyclonal at 1/20000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

ab7028 staining HDAC1 in human primary cardiac cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with permeabilization buffer. The sample was incubated with the undiluted primary antibody for 12 hours at 4°C. An Allophycocyanin-conjugated goat polyclonal anti-rabbit IgG (1/1000) was used as the secondary antibody.Gating Strategy: Isotype negative control (white).See Abreview

HDAC1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to HDAC1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab7028.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 60ka: HDAC1.

Product References

Analysis of the SWI/SNF chromatin-remodeling complex during early heart - Analysis of the SWI/SNF chromatin-remodeling complex during early heart

Singh AP, Archer TK. Nucleic Acids Res. 2014 Mar;42(5):2958-75.

A single allele of Hdac2 but not Hdac1 is sufficient for normal mouse brain - A single allele of Hdac2 but not Hdac1 is sufficient for normal mouse brain

Hagelkruys A, Lagger S, Krahmer J, Leopoldi A, Artaker M, Pusch O, Zezula J, Weissmann S, Xie Y, Schofer C, Schlederer M, Brosch G, Matthias P, Selfridge J, Lassmann H, Knoblich JA, Seiser C. Development. 2014 Feb;141(3):604-16.

In vivo transcriptional governance of hair follicle stem cells by canonical Wnt - In vivo transcriptional governance of hair follicle stem cells by canonical Wnt

Lien WH, Polak L, Lin M, Lay K, Zheng D, Fuchs E. Nat Cell Biol. 2014 Feb;16(2):179-90.

A novel protein, CHRONO, functions as a core component of the mammalian circadian - A novel protein, CHRONO, functions as a core component of the mammalian circadian

Goriki A, Hatanaka F, Myung J, Kim JK, Yoritaka T, Tanoue S, Abe T, Kiyonari H, Fujimoto K, Kato Y, Todo T, Matsubara A, Forger D, Takumi T. PLoS Biol. 2014 Apr 15;12(4):e1001839.

Global gene repression by the steroid receptor coactivator SRC-1 promotes - Global gene repression by the steroid receptor coactivator SRC-1 promotes

Walsh CA, Bolger JC, Byrne C, Cocchiglia S, Hao Y, Fagan A, Qin L, Cahalin A, McCartan D, McIlroy M, O'Gaora P, Xu J, Hill AD, Young LS. Cancer Res. 2014 May 1;74(9):2533-44.

Epigenetic modification of the leptin promoter in diet-induced obese mice and the - Epigenetic modification of the leptin promoter in diet-induced obese mice and the

Shen W, Wang C, Xia L, Fan C, Dong H, Deckelbaum RJ, Qi K. Sci Rep. 2014 Jun 13;4:5282.

Loss of Prox1 in striated muscle causes slow to fast skeletal muscle fiber - Loss of Prox1 in striated muscle causes slow to fast skeletal muscle fiber

Petchey LK, Risebro CA, Vieira JM, Roberts T, Bryson JB, Greensmith L, Lythgoe MF, Riley PR. Proc Natl Acad Sci U S A. 2014 Jul 1;111(26):9515-20. doi:

c-Myc-induced transcription factor AP4 is required for host protection mediated - c-Myc-induced transcription factor AP4 is required for host protection mediated

Chou C, Pinto AK, Curtis JD, Persaud SP, Cella M, Lin CC, Edelson BT, Allen PM, Colonna M, Pearce EL, Diamond MS, Egawa T. Nat Immunol. 2014 Sep;15(9):884-93.

Ectopic DNMT3L triggers assembly of a repressive complex for retroviral silencing - Ectopic DNMT3L triggers assembly of a repressive complex for retroviral silencing

Kao TH, Liao HF, Wolf D, Tai KY, Chuang CY, Lee HS, Kuo HC, Hata K, Zhang X, Cheng X, Goff SP, Ooi SK, Bestor TH, Lin SP. J Virol. 2014 Sep;88(18):10680-95.

Prohibitin is required for transcriptional repression by the WT1-BASP1 complex. - Prohibitin is required for transcriptional repression by the WT1-BASP1 complex.

Toska E, Shandilya J, Goodfellow SJ, Medler KF, Roberts SG. Oncogene. 2014 Oct 23;33(43):5100-8.