![Overlay histogram showing K562 cells stained with ab77125 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77125, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/29860_Hemoglobin-Primary-antibodies-ab77125-1.jpg)
Overlay histogram showing K562 cells stained with ab77125 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77125, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
![All lanes : Anti-Hemoglobin antibody [7E1F] (ab77125) at 1/1000 dilutionLane 1 : Hemoglobin isolated from Human plasmaLane 2 : Human plasma at 1 µl](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/29859_ab77125.jpg)
All lanes : Anti-Hemoglobin antibody [7E1F] (ab77125) at 1/1000 dilutionLane 1 : Hemoglobin isolated from Human plasmaLane 2 : Human plasma at 1 µl