Anti-Hexokinase 1 antibody
| Name | Anti-Hexokinase 1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab55144 |
| Prices | $390.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | WB IHC-P ICC/IF ICC/IF FC |
| Species Reactivities | Human |
| Antigen | Recombinant fragment: NQNVHMESEV YDTPENIVHG SGSQLFDHVA ECLGDFMEKR KIKDKKLPVG FTFSFPCQQS KIDEAILITW TKRFKASGVE GADVVKLLNK AIKKRGDYDA , corresponding to amino acids 101-201 of Human Hexokinase Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | HK1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Hexokinase antibody (ab55144) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human salivary gland.
ICC/IF image of ab55144 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55144, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Overlay histogram showing K562 cells stained with ab55144 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55144, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/210_Hexokinase-1-Primary-antibodies-ab55144-2.jpg)
Overlay histogram showing K562 cells stained with ab55144 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55144, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Biological correlation of (1)(8)F-FDG uptake on PET in pulmonary neuroendocrine - Biological correlation of (1)(8)F-FDG uptake on PET in pulmonary neuroendocrine
Kaira K, Murakami H, Endo M, Ohde Y, Naito T, Kondo H, Nakajima T, Yamamoto N, Takahashi T. Anticancer Res. 2013 Oct;33(10):4219-28.
Depolarized MUC1 expression is closely associated with hypoxic markers and poor - Depolarized MUC1 expression is closely associated with hypoxic markers and poor
Kaira K, Nakagawa K, Ohde Y, Okumura T, Takahashi T, Murakami H, Endo M, Kondo H, Nakajima T, Yamamoto N. Int J Surg Pathol. 2012 Jun;20(3):223-32.
LAT1 expression is closely associated with hypoxic markers and mTOR in resected - LAT1 expression is closely associated with hypoxic markers and mTOR in resected
Kaira K, Oriuchi N, Takahashi T, Nakagawa K, Ohde Y, Okumura T, Murakami H, Shukuya T, Kenmotsu H, Naito T, Kanai Y, Endo M, Kondo H, Nakajima T, Yamamoto N. Am J Transl Res. 2011;3(5):468-78. Epub 2011 Oct 7.