Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker

• Supplier: Abcam
• Catalog: ab150423
• Prices: $99.00
• Sizes: 10 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR10134(B)
• Applications: WB IHC-P ICC/IF ICC/IF FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence)
• Description: Rabbit Monoclonal
• Gene: HK1
• Conjugate: Unconjugated

Product images

Overlay histogram showing K562 cells stained with ab150423 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab150423, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab150423) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : MCF-7 cell lysateLysates/proteins at 10 µg per lane.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Hexokinase 1 with ab150423 at 1/50 dilution.

Immunofluorescent analysis of 293 cells labelling Hexokinase 1 with ab150423 at 1/100 dilution.