Anti-HGF antibody
| Name | Anti-HGF antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab83760 |
| Prices | $390.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF WB ELISA |
| Species Reactivities | Mouse, Rat, Human, Sheep, Rabbit, Horse, Guinea Pig, Bovine, Cat, Dog, Pig |
| Antigen | A synthetic peptide corresponding to a region within the N-terminal sequence 108-157 (VKKEFGHEFD LYENKDYIRN CIIGKGRSYK GTVSITKSGI KCQPWSSMIP) of Human HGF, NP_001010932 |
| Description | Rabbit Polyclonal |
| Gene | HGF |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-HGF antibody (ab83760) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution
ab83760 staining HGF in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100) for 32 minutes at 37°C. An undiluted biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview
Immunohistochemistry of Human Adrenal Gland tissue at an antibody concentration of 5.0µg/ml using anti-HGF antibody (ab83760)
ICC/IF image of ab83760 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83760, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Conditional genetic elimination of hepatocyte growth factor in mice compromises - Conditional genetic elimination of hepatocyte growth factor in mice compromises
Nejak-Bowen K, Orr A, Bowen WC Jr, Michalopoulos GK. PLoS One. 2013;8(3):e59836.
Lymphatic function is regulated by a coordinated expression of lymphangiogenic - Lymphatic function is regulated by a coordinated expression of lymphangiogenic
Zampell JC, Avraham T, Yoder N, Fort N, Yan A, Weitman ES, Mehrara BJ. Am J Physiol Cell Physiol. 2012 Jan 15;302(2):C392-404. doi:
Distinct interactions between c-Src and c-Met in mediating resistance to c-Src - Distinct interactions between c-Src and c-Met in mediating resistance to c-Src
Sen B, Peng S, Saigal B, Williams MD, Johnson FM. Clin Cancer Res. 2011 Feb 1;17(3):514-24.
Autocrine-controlled formation and function of tissue-like aggregates by primary - Autocrine-controlled formation and function of tissue-like aggregates by primary
Williams CM, Mehta G, Peyton SR, Zeiger AS, Van Vliet KJ, Griffith LG. Tissue Eng Part A. 2011 Apr;17(7-8):1055-68.