All lanes : Anti-HIF1 beta antibody [1D1] (ab118142) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY HIF1 beta cDNALysates/proteins at 5 µg per lane.
ab118142, at a 1/100 dilution, staining HIF1 beta in COS7 cells transiently transfected with pCMV6-ENTRY HIF1 beta by Immunofluorescene.
Flow cytometric analysis of Hela cells, using ab118142 at a 1/100 dilution (Red), compared to a non-specific negative control antibody (Blue).
Flow cytometric analysis of Jurkat cells, using ab118142 at a 1/100 dilution (Red), compared to a non-specific negative control antibody (Blue).