All lanes : Anti-Histone H2A (acetyl K13) antibody (ab176427) at 1 µg/mlLane 1 : HeLa Sodium Butyrate-treated 0uM Control (Cell acid extract ) at 10 µgLane 2 : HeLa Sodium Butyrate-treated 10uM (Cell acid extract ) at 10 µgLane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µgLane 4 : Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µgSecondaryGoat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution
ab176427 staining Histone H2A type 1-B/E (Acetylation K13) in HeLa cells. The cells were fixed with 100% methanol (5 min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab176427 at 1ug/ml and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594) at 2µg/ml (Shown in red). This was followed by an incubation at room temperature for 1h with ab150081,Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).