Anti-Histone H2A antibody [EPR17470] - C-terminal

• Supplier: Abcam
• Catalog: ab177308
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR17470
• Applications: ICC/IF ICC/IF IHC-P WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Histone H2A aa 50 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: HIST1H2AE
• Conjugate: Unconjugated

Product images

Anti-Histone H2A antibody [EPR17470] - C-terminal (ab177308) at 1/5000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-Histone H2A antibody [EPR17470] - C-terminal (ab177308) at 1/1000 dilutionLane 1 : mouse brain lysateLane 2 : mouse heart lysateLane 3 : mouse kidney lysateLane 4 : mouse spleen lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Histone H2A using ab177308 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstained with Hematoxylin. Nucleus staining on epithelial cells of Human colon tissue was observed.Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Histone H2A using ab177308 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstained with Hematoxylin. Nucleus staining on epithelial cells of mouse colon tissue was observed.Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution..

Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Histone H2A using ab177308 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstained with Hematoxylin. Nucleus staining on epithelial cells of rat colon tissue was observed.Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Histone H2A with ab177308 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).Confocal image showing nuclear staining on HeLa cell line.The nuclear counter stain is DAPI (blue).Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:1. ab177308 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.