Anti-Histone H2A.X (phospho S139) antibody [EP854(2)Y] - ChIP Grade

• Supplier: Abcam
• Catalog: ab81299
• Prices: $400.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP854(2)Y
• Applications: WB IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: A synthetic phospho-peptide corresponding to residues surrounding serine 139 of Human Histone H2A
• Description: Rabbit Monoclonal
• Gene: H2AFX
• Conjugate: Unconjugated

Product images

Immunohistochemical staining of paraffin embedded human brain with purified ab81299 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

All lanes : Anti-Histone H2A.X (phospho S139) antibody [EP854(2)Y] - ChIP Grade (ab81299) at 1/10000 dilution (purified)Lane 1 : Untreated HepG2 cell lysateLane 2 : HepG2 cell lysate treated with etoposideLysates/proteins at 10 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

IHC-P image of γH2A.X staining on Mouse testis sections using unpurified ab81299 (1:5000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab81299 was diluted 1:5000 in TBS buffer (containing BSA and Azide) and sections were then incubated with unpurified ab81299 for 2 hours at 21°C. The secondary antibody used was Biotin conjugated Goat polyclonal to Rabbit IgG (1:250).See Abreview

IHC-P image of γH2A.X staining on Rat testis sections using unpurified ab81299 (1:5000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab81299 was diluted 1:5000 in TBS buffer (containing BSA and Azide) and sections were then incubated with unpurified ab81299 for 2 hours at 21°C. The secondary antibody used was Biotin conjugated Goat polyclonal to Rabbit IgG (1:250).See Abreview

Immunohistochemical staining of paraffin embedded human brain with unpurified ab81299 at a working dilution of 1 in 50. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

All lanes : Anti-Histone H2A.X (phospho S139) antibody [EP854(2)Y] - ChIP Grade (ab81299) at 1/5000 dilution (purified)Lane 1 : Untreated HepG2 cell lysateLane 2 : HepG2 cell lysate treated with etoposideLysates/proteins at 10 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

All lanes : Anti-Histone H2A.X (phospho S139) antibody [EP854(2)Y] - ChIP Grade (ab81299) at 1/500000 dilution (unpurified)Lane 1 : Jurkat cell lysatesLane 2 : Jurkat cell lysates treated with etoposideLysates/proteins at 10 µg per lane.SecondaryHRP Labelled goat anti-rabbit at 1/2000 dilution

Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human kidney transitional cell carcinoma using unpurified ab81299 at a dilution of 1/100.

Product References

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