Overlay histogram showing HeLa cells stained with ab52599 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52599, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![Anti-Histone H2B antibody [EP957Y] (ab52599) at 1/20000 dilution + A431 cell lysate at 10 µgSecondaryHRP-labelled goat anti-rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/1428_ab52599wb.jpg)
Anti-Histone H2B antibody [EP957Y] (ab52599) at 1/20000 dilution + A431 cell lysate at 10 µgSecondaryHRP-labelled goat anti-rabbit at 1/2000 dilution
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab52599 at a dilution of 1/250-1/500.
Fluorescent staining of HeLa cells using ab52599 at a dilution of 1/50-1/100.