Anti-Histone H3 (acetyl K122) antibody - ChIP Grade
| Name | Anti-Histone H3 (acetyl K122) antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab33309 |
| Prices | $380.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP ChIP ChIPseq WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human, Arabidopsis thaliana, Drosophila, Broad species |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human Histone H3, acetylated at K122 |
| Blocking Peptide | Human Histone H3 (acetyl K122) peptide |
| Description | Rabbit Polyclonal |
| Gene | HIST1H3A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Histone H3 (acetyl K122) antibody - ChIP Grade (ab33309) at 1 µg/mlLane 1 : HeLa Histone Preparation Nuclear Lysate - Butyrated Lane 2 : HeLa Histone Preparation Nuclear Lysate - Butyrated with Human Histone H3 (acetyl K122) peptide (ab34466) at 1 µg/mlLane 3 : HeLa Histone Preparation Nuclear Lysate - Butyrated with Histone H3 peptide - unmodified (ab34467) at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
ICC/IF image of ab33309 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab33309, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).