Anti-Histone H3 (acetyl K9) antibody [Y28]

• Supplier: Abcam
• Catalog: ab32129
• Prices: $391.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: Y28
• Applications: WB IHC-P ICC/IF IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: A synthetic acetylated peptide corresponding to residues surrounding Lys9 of Histone H3
• Description: Rabbit Monoclonal
• Gene: H3F3A
• Conjugate: Unconjugated

Product images

IHC image of ab32129 staining Histone H3 (acetyl K9) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32129, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

All lanes : Anti-Histone H3 (acetyl K9) antibody [Y28] (ab32129) at 1/500 dilutionLane 1 : Untreated NIH 3T3 cell lysateLane 2 : NIH 3T3 cell lysate treated with TSA

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using ab32129 at 1/50 - 1/100Immunohistochemical analysis of paraffin-embedded human lung carcinoma using ab32129 at 1/50 - 1/100

Product References

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