Anti-Histone H3 (di methyl K79) antibody [EPR17467]

• Supplier: Abcam
• Catalog: ab177184
• Prices: $384.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR17467
• Applications: IHC-P WB ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)
• Description: Rabbit Monoclonal
• Gene: HIST3H3
• Conjugate: Unconjugated

Product images

All lanes : Anti-Histone H3 (di methyl K79) antibody [EPR17467] (ab177184) at 1/2500 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLane 2 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Histone H3 (di methyl K79) with ab177184 at 1/2000 dilution, followed by Goat anti-rabbit Alexa Fluor® 488 (ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on HeLa cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (goat anti-mouse AlexaFluor®594 secondary) at 1/500 dilution (red).The negative controls are as follows;1. ab177184 at 1/2000 dilution followed by ab150120 (goat anti-mouse AlexaFluor®594 secondary) at 1/500 dilution.2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (goat anti-rabbit Alexa Fluor®488; IgG H&L) at 1/400 dilution.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Histone H3 (di methyl K79) with ab177184 at 1/2000 dilution, followed by Goat Anti-Rabbit HRP (IgG H&L) at 1/500 dilution (ab97051). Nucleus staining on glandular epithelium of Human colon tissue is observed. Counter stained with Hematoxylin.Negative control: Using PBS instead of primary antibody.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Histone H3 (di methyl K79) with ab177184 at 1/2000 dilution, followed by Goat Anti-Rabbit HRP (IgG HRP) at 1/500 dilution (ab97051). Nucleus staining mouse liver tissue is observed. Counter stained with Hematoxylin.Negative control: Using PBS instead of primary antibody.

Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling Histone H3 (di methyl K79) with ab177184 at 1/2000 dilution, followed by Goat Anti-Rabbit HRP (IgG H&L) at 1/500 dilution (ab97051). Nucleus staining on rat pancreas tissue is observed. Counter stained with Hematoxylin.Negative control: Using PBS instead of primary antibody.