Anti-Histone H3 (phospho S10) antibody [EPR17246] - ChIP Grade

• Supplier: Abcam
• Catalog: ab177218
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR17246
• Applications: ChIP IHC-P WB ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Histone H3 aa 1-100 (phospho S10)
• Description: Rabbit Monoclonal
• Gene: H3F3A
• Conjugate: Unconjugated

Product images

All lanes : Anti-Histone H3 (phospho S10) antibody [EPR17246] - ChIP Grade (ab177218) at 1/200 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) treated with 1.5µg/ml Colcemid for 12 hours whole cell lysatesLane 2 : Untreated HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysatesLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-Histone H3 (phospho S10) antibody [EPR17246] - ChIP Grade (ab177218) at 1/200 dilutionLane 1 : NIH/3T3 (Mouse embyro fibroblast cells) treated with 1.5µg/ml Colcemid for 12 hours whole cell lysatesLane 2 : Untreated NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysatesLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Histone H3 (phospho S10) with ab177218 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on Human placenta tissue is observed. Counter stained with Hematoxylin.Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Histone H3 (phospho S10) with ab177218 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on mouse colon tissue is observed. Counter stained with Hematoxylin.Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Histone H3 (phospho S10) with ab177218 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on rat colon tissue is observed. Counter stained with Hematoxylin.Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Histone H3 (phospho S10) with ab177218 at 1/5000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:-ve control 1: ab177218 at 1/5000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

Chromatin was prepared from HeLa (Human epithelial cells from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. HeLa (Human epithelial cells from cervix adenocarcinoma) cells were treated with colcemid at 150ng/ml for 20 h. Treated and non-treated cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab177218 (blue), and 20µl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).