Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] - ChIP Grade (Alexa Fluor® 488)
| Name | Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] - ChIP Grade (Alexa Fluor® 488) |
|---|---|
| Supplier | Abcam |
| Catalog | ab197502 |
| Prices | $425.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | mAbcam 14955 |
| Applications | ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse, Rat, Chicken, Yeast, Xenopus, Arabidopsis thaliana, C. elegans, Drosophila, Deer, S. pombe, Zebrafish, Mammalian, Tobacco, Chlamydomonas reinhardtii, Monkey, Aspergillus, Neurospora crassa |
| Antigen | Phospho S10 specific clone produced using a synthetic peptide derived from residues 1 - 100 of Human Histone H3, phosphorylated at S10 and tri methylated at K9 |
| Description | Mouse Monoclonal |
| Gene | CELE_F58A4.3 |
| Conjugate | Alexa Fluor® 488 |
| Supplier Page | Shop |
Product images
ab197502 staining Histone H3 (phospho S10) in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197502 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).