All lanes : Anti-Histone H3 (phospho S28) antibody (ab183767) at 1/1000 dilutionLane 1 : HeLa whole cell lysate (untreated)Lane 2 : HeLa whole cell lysate (100 ng/ml Nocodazole treatment for 24 hr)Lysates/proteins at 30 µg per lane.
Immunofluorescent analysis of asynchronous HeLa cells (fixed in 2% paraformaldehyde for 30 mins) labeling Histone H3 (phospho S28) at 1/500 dilution (green). Counter stained with Hoechst 33342 (blue) in bottom panels.
Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling Histone H3 (phospho S28) with ab183767 at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Histone H3 (phospho S28) with ab183767 at 1/500 dilution.
Dot blot analysis labeling Histone H3 (phospho S28).Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with ab183767 at 1/5000 dilution.1: Peptide samples of H3S10 un2: Peptide samples of H3S10ph3: Peptide samples of H3S28 un4: Peptide samples of H3S28ph