Anti-Histone H3.3 antibody - ChIP Grade
| Name | Anti-Histone H3.3 antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab62642 |
| Prices | $391.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA ICC/IF ICC/IF IHC-P WB ChIP |
| Species Reactivities | Rat, Human |
| Antigen | Synthetic peptide corresponding to Human Histone H3 |
| Description | Rabbit Polyclonal |
| Gene | H3F3A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunofluorescence analysis of HeLa cells, using ab62642 at a 1/500 dilution. Left image untreated. Right image treated with peptide.
Immunohistochemistry analysis of paraffin embedded human brain tissue using ab62642 at a 1/50 dilution. Left image untreated. Right image treated with peptide.
ChIP of Histone H3.3 from primary adipose chromatin using ab62642, showing enrichment at promoter regions previously associated with Histone H3.3 enrichment. The ChIP experiment was performed according to Dahl and Collas, 2007 (please see accompanying abreview for further details). See Abreview
Anti-Histone H3.3 antibody - ChIP Grade (ab62642) at 1 µg/ml + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
The proteomic investigation of chromatin functional domains reveals novel - The proteomic investigation of chromatin functional domains reveals novel
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Control of human adenovirus type 5 gene expression by cellular Daxx/ATRX - Control of human adenovirus type 5 gene expression by cellular Daxx/ATRX
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Accumulation of the FACT complex, as well as histone H3.3, serves as a target - Accumulation of the FACT complex, as well as histone H3.3, serves as a target
Aida M, Hamad N, Stanlie A, Begum NA, Honjo T. Proc Natl Acad Sci U S A. 2013 May 7;110(19):7784-9. doi:
Histone variant H3.3 maintains a decondensed chromatin state essential for mouse - Histone variant H3.3 maintains a decondensed chromatin state essential for mouse
Lin CJ, Conti M, Ramalho-Santos M. Development. 2013 Sep;140(17):3624-34.
Mouse BAZ1A (ACF1) is dispensable for double-strand break repair but is essential - Mouse BAZ1A (ACF1) is dispensable for double-strand break repair but is essential
Dowdle JA, Mehta M, Kass EM, Vuong BQ, Inagaki A, Egli D, Jasin M, Keeney S. PLoS Genet. 2013 Nov;9(11):e1003945.
Heterochromatin protein 1 gamma and IkappaB kinase alpha interdependence during - Heterochromatin protein 1 gamma and IkappaB kinase alpha interdependence during
Thorne JL, Ouboussad L, Lefevre PF. Nucleic Acids Res. 2012 Sep;40(16):7676-89.
Dissecting epigenetic silencing complexity in the mouse lung cancer suppressor - Dissecting epigenetic silencing complexity in the mouse lung cancer suppressor
Reamon-Buettner SM, Borlak J. PLoS One. 2012;7(6):e38531.
Synergic reprogramming of mammalian cells by combined exposure to mitotic Xenopus - Synergic reprogramming of mammalian cells by combined exposure to mitotic Xenopus
Ganier O, Bocquet S, Peiffer I, Brochard V, Arnaud P, Puy A, Jouneau A, Feil R, Renard JP, Mechali M. Proc Natl Acad Sci U S A. 2011 Oct 18;108(42):17331-6. doi: