Anti-HIV1 p17 antibody [2D11]
| Name | Anti-HIV1 p17 antibody [2D11] |
|---|---|
| Supplier | Abcam |
| Catalog | ab9067 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 2D11 |
| Applications | FC WB ELISA ICC/IF ICC/IF Radioimmunoprecipitation |
| Description | Mouse Monoclonal |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab9067 staining HIV1 p17 (red) in Jurkat cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized. The sample was incubated with the primary antibody (1/100 in PBS + 10% FBS) for 30 minutes at 4°C. An undiluted phycoerythrin-conjugated goat anti-mouse IgG was used as the secondary antibody. Gating Strategy: Live Cells.See Abreview
Flow cytometric analysis of monocyte-derived macrophages infected with HIV, staining HIV1 p17 with ab9067. Cells were treated with 1a,25-dihydroxycholecalciferol (1,25D3) at varying concentrations, before being infected with HIV.
Immunofluorescence analysis of Macrophages treated with 100 pM 1,25D3 or vehicle control (EtOH) and infected with HIV1Ba-L, staining HIV1 p17 with ab9067. Cells were fixed and permeabilized in PBS supplemented with paraformaldehyde and 0.1% saponin for 30 min. Samples were incubated with primary antibody and an AlexaFluor®488-conjugated goat anti-mouse IgG was used as the secondary antibody.
Product References
Vitamin D inhibits human immunodeficiency virus type 1 and Mycobacterium - Vitamin D inhibits human immunodeficiency virus type 1 and Mycobacterium
Campbell GR, Spector SA. PLoS Pathog. 2012;8(5):e1002689.
Hormonally active vitamin D3 (1alpha,25-dihydroxycholecalciferol) triggers - Hormonally active vitamin D3 (1alpha,25-dihydroxycholecalciferol) triggers
Campbell GR, Spector SA. J Biol Chem. 2011 May 27;286(21):18890-902.
Autophagy pathway intersects with HIV-1 biosynthesis and regulates viral yields - Autophagy pathway intersects with HIV-1 biosynthesis and regulates viral yields
Kyei GB, Dinkins C, Davis AS, Roberts E, Singh SB, Dong C, Wu L, Kominami E, Ueno T, Yamamoto A, Federico M, Panganiban A, Vergne I, Deretic V. J Cell Biol. 2009 Jul 27;186(2):255-68.