Anti-HLA A antibody [EP1395Y]

• Supplier: Abcam
• Catalog: ab52922
• Prices: $401.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1395Y
• Applications: IHC-F IHC ELISA WB IP FC IHC-P ICC/IF ICC/IF
• Species Reactivities: Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MHC Class I alpha aa 50-150
• Description: Rabbit Monoclonal
• Gene: HLA-A
• Conjugate: Unconjugated

Product images

Anti-HLA A antibody [EP1395Y] (ab52922) at 1/10000 dilution + Raji cell lysate at 10 µgSecondaryGoat anti rabbit IgG HRP conjugated at 1/2000 dilution

ab52922 staining HLA A in Human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% H2O2 for 10 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer, pH 6.0 . Samples were incubated with primary antibody (1/3000) for 20 minutes at 25°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview

IHC - Wholemount of human zebrafish xenograft labelling HLA A with ab52922. Sample was incubated with primary antibody (1/100) for 1 hours at 4°C. An Alexa Flour® 647-conjugated goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.See Abreview

ICC/IF image of ab52922 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52922, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Overlay histogram showing Raji cells stained with ab52922 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52922, 1/100) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Raji cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Product References

Arresting a Torsin ATPase reshapes the endoplasmic reticulum. - Arresting a Torsin ATPase reshapes the endoplasmic reticulum.

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Epidermal growth factor receptor inhibition augments the expression of MHC class - Epidermal growth factor receptor inhibition augments the expression of MHC class

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All 4 di-leucine motifs in the first hydrophobic domain of the E5 oncoprotein of - All 4 di-leucine motifs in the first hydrophobic domain of the E5 oncoprotein of

Cortese MS, Ashrafi GH, Campo MS. Int J Cancer. 2010 Apr 1;126(7):1675-82.