Anti-HLA E antibody [MEM-E/02]
| Name | Anti-HLA E antibody [MEM-E/02] |
|---|---|
| Supplier | Abcam |
| Catalog | ab2216 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | MEM-E/02 |
| Applications | FC IHC-F WB IHC-P |
| Species Reactivities | Human, Monkey |
| Antigen | Recombinant denatured heavy chain (Human) |
| Description | Mouse Monoclonal |
| Gene | HLA-E |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of ab2216 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2216, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab2216 staining HLA E in Human brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was permeabilized with methanol + 1% H2O2. Samples were incubated with primary antibody (1/100 in 4% serum + 1% BSA + 0.01% azide) for 12 hours. A Biotin-conjugated Horse anti-mouse IgG monoclonal (1/100) was used as the secondary antibody.See Abreview
![Anti-HLA E antibody [MEM-E/02] (ab2216) at 1/6000 dilution + whole cell lysate prepared from human endothelial cell line at 100 µgSecondaryHRP conjugated goat anti-mouse polyclonal at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/2665_HLA-E-Primary-antibodies-ab2216-13.jpg)
Anti-HLA E antibody [MEM-E/02] (ab2216) at 1/6000 dilution + whole cell lysate prepared from human endothelial cell line at 100 µgSecondaryHRP conjugated goat anti-mouse polyclonal at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Immunohistochemical analysis of frozen Human adult temporal lobe sections, staining HLA E with ab2216, at 1/20 dilution. Arrows denote the location of microglia (M) and neurons (N).
![Overlay histogram showing HL60 cells stained with ab2216 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2216, 1 µg/1x106 cells for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/2667_HLA-E-Primary-antibodies-ab2216-15.jpg)
Overlay histogram showing HL60 cells stained with ab2216 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2216, 1 µg/1x106 cells for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Combined analysis of HLA class I, HLA-E and HLA-G predicts prognosis in colon - Combined analysis of HLA class I, HLA-E and HLA-G predicts prognosis in colon
Zeestraten EC, Reimers MS, Saadatmand S, Goossens-Beumer IJ, Dekker JW, Liefers GJ, van den Elsen PJ, van de Velde CJ, Kuppen PJ. Br J Cancer. 2014 Jan 21;110(2):459-68.
Immunological subtypes in breast cancer are prognostic for invasive ductal but - Immunological subtypes in breast cancer are prognostic for invasive ductal but
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Prognostic value of HLA class I, HLA-E, HLA-G and Tregs in rectal cancer: a - Prognostic value of HLA class I, HLA-E, HLA-G and Tregs in rectal cancer: a
Reimers MS, Engels CC, Putter H, Morreau H, Liefers GJ, van de Velde CJ, Kuppen PJ. BMC Cancer. 2014 Jul 5;14:486.
Cytotoxic NKG2C+ CD4 T cells target oligodendrocytes in multiple sclerosis. - Cytotoxic NKG2C+ CD4 T cells target oligodendrocytes in multiple sclerosis.
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Infection of human endothelial cells by Japanese encephalitis virus: increased - Infection of human endothelial cells by Japanese encephalitis virus: increased
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Dynamic gene expression in the human cerebral cortex distinguishes children from - Dynamic gene expression in the human cerebral cortex distinguishes children from
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Herpes B virus, macacine herpesvirus 1, breaks simplex virus tradition via major - Herpes B virus, macacine herpesvirus 1, breaks simplex virus tradition via major
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HLA-E expression by gynecological cancers restrains tumor-infiltrating CD8(+) T - HLA-E expression by gynecological cancers restrains tumor-infiltrating CD8(+) T
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The HCMV membrane glycoprotein US10 selectively targets HLA-G for degradation. - The HCMV membrane glycoprotein US10 selectively targets HLA-G for degradation.
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HLA-E and HLA-G expression in classical HLA class I-negative tumors is of - HLA-E and HLA-G expression in classical HLA class I-negative tumors is of
de Kruijf EM, Sajet A, van Nes JG, Natanov R, Putter H, Smit VT, Liefers GJ, van den Elsen PJ, van de Velde CJ, Kuppen PJ. J Immunol. 2010 Dec 15;185(12):7452-9.