Anti-hnRNP K antibody [3C2] - ChIP Grade
| Name | Anti-hnRNP K antibody [3C2] - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab39975 |
| Prices | $401.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | 3C2 |
| Applications | WB IP ELISA ICC/IF ICC/IF FC ChIP ChIP |
| Species Reactivities | Mouse, Rat, Hamster, Bovine, Human, Xenopus |
| Antigen | Full length native protein (purified) (Human) |
| Description | Mouse Monoclonal |
| Gene | HNRNPK |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab39975 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab39975 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![All lanes : Anti-hnRNP K antibody [3C2] - ChIP Grade (ab39975) at 1/1000 dilutionLane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/3381_hnRNP-K-Primary-antibodies-ab39975-2.jpg)
All lanes : Anti-hnRNP K antibody [3C2] - ChIP Grade (ab39975) at 1/1000 dilutionLane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
![Overlay histogram showing HeLa cells stained with ab39975 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab39975, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/3382_hnRNP-K-Primary-antibodies-ab39975-14.jpg)
Overlay histogram showing HeLa cells stained with ab39975 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab39975, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
hnRNP K coordinates transcriptional silencing by SETDB1 in embryonic stem cells. - hnRNP K coordinates transcriptional silencing by SETDB1 in embryonic stem cells.
Thompson PJ, Dulberg V, Moon KM, Foster LJ, Chen C, Karimi MM, Lorincz MC. PLoS Genet. 2015 Jan 22;11(1):e1004933.
A human proteome microarray identifies that the heterogeneous nuclear - A human proteome microarray identifies that the heterogeneous nuclear
Fan B, Lu KY, Reymond Sutandy FX, Chen YW, Konan K, Zhu H, Kao CC, Chen CS. Mol Cell Proteomics. 2014 Jan;13(1):84-92.
An RNAi screen for Aire cofactors reveals a role for Hnrnpl in polymerase release - An RNAi screen for Aire cofactors reveals a role for Hnrnpl in polymerase release
Giraud M, Jmari N, Du L, Carallis F, Nieland TJ, Perez-Campo FM, Bensaude O, Root DE, Hacohen N, Mathis D, Benoist C. Proc Natl Acad Sci U S A. 2014 Jan 28;111(4):1491-6. doi:
Cellular RNA binding proteins NS1-BP and hnRNP K regulate influenza A virus RNA - Cellular RNA binding proteins NS1-BP and hnRNP K regulate influenza A virus RNA
Tsai PL, Chiou NT, Kuss S, Garcia-Sastre A, Lynch KW, Fontoura BM. PLoS Pathog. 2013;9(6):e1003460.
A large intergenic noncoding RNA induced by p53 mediates global gene repression - A large intergenic noncoding RNA induced by p53 mediates global gene repression
Huarte M, Guttman M, Feldser D, Garber M, Koziol MJ, Kenzelmann-Broz D, Khalil AM, Zuk O, Amit I, Rabani M, Attardi LD, Regev A, Lander ES, Jacks T, Rinn JL. Cell. 2010 Aug 6;142(3):409-19.