Anti-Ikaros antibody

• Supplier: Abcam
• Catalog: ab26083
• Prices: $390.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-P WB ICC/IF ICC/IF
• Species Reactivities: Mouse, Human, Chicken
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Ikaros
• Description: Rabbit Polyclonal
• Gene: IKZF1
• Conjugate: Unconjugated

Product images

All lanes : Anti-Ikaros antibody (ab26083) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : Ramos (Human Burkitt's lymphoma cell line)Whole Cell LysateLane 4 : Spleen (Human) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

IHC image of Ikaros staining in human tonsil FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab26083, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-Ikaros antibody (ab26083) at 1 µg/ml + MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µgSecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

ICC/IF image of ab26083 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab26083 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Ikaros imposes a barrier to CD8+ T cell differentiation by restricting autocrine - Ikaros imposes a barrier to CD8+ T cell differentiation by restricting autocrine

O'Brien S, Thomas RM, Wertheim GB, Zhang F, Shen H, Wells AD. J Immunol. 2014 Jun 1;192(11):5118-29.

Ikaros deletions in BCR-ABL-negative childhood acute lymphoblastic leukemia are - Ikaros deletions in BCR-ABL-negative childhood acute lymphoblastic leukemia are

Vitanza NA, Zaky W, Blum R, Meyer JA, Wang J, Bhatla T, Morrison DJ, Raetz EA, Carroll WL. Pediatr Blood Cancer. 2014 Oct;61(10):1779-85.

HDAC7 is a repressor of myeloid genes whose downregulation is required for - HDAC7 is a repressor of myeloid genes whose downregulation is required for

Barneda-Zahonero B, Roman-Gonzalez L, Collazo O, Rafati H, Islam AB, Bussmann LH, di Tullio A, De Andres L, Graf T, Lopez-Bigas N, Mahmoudi T, Parra M. PLoS Genet. 2013 May;9(5):e1003503.

Ikaros is required to survive positive selection and to maintain clonal diversity - Ikaros is required to survive positive selection and to maintain clonal diversity

Tinsley KW, Hong C, Luckey MA, Park JY, Kim GY, Yoon HW, Keller HR, Sacks AJ, Feigenbaum L, Park JH. Blood. 2013 Oct 3;122(14):2358-68.

Ikaros is degraded by proteasome-dependent mechanism in the early phase of - Ikaros is degraded by proteasome-dependent mechanism in the early phase of

He LC, Xu HZ, Gu ZM, Liu CX, Chen GQ, Wang YF, Wen DH, Wu YL. Biochem Biophys Res Commun. 2011 Mar 18;406(3):430-4. doi: