Anti-IKZF3 antibody [EPR9342(B)]

• Supplier: Abcam
• Catalog: ab139408
• Prices: $381.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR9342(B)
• Applications: WB IHC-P ICC/IF ICC/IF FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human IKZF3 aa 1-100 (N terminal)
• Description: Rabbit Monoclonal
• Gene: IKZF3
• Conjugate: Unconjugated

Product images

All lanes : Anti-IKZF3 antibody [EPR9342(B)] (ab139408) at 1/20000 dilution (purified)Lane 1 : Raji cell lysateLane 2 : Ramos cell lysateLane 3 : Jurkat cell lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-IKZF3 antibody [EPR9342(B)] (ab139408) at 1/1000 dilution (unpurified)Lane 1 : Human thymus cell lysateLane 2 : Jurkat cell lysateLane 3 : Raji cell lysateLane 4 : Ramos cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling IKZF3 with purified ab139408 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue labelling IKZF3 with purified ab139408 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue labelling IKZF3 with purified ab139408 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling IKZF3 with unpurified ab139408 at 1/250 dilution.

Immunocytochemistry/Immunofluorescence analysis of Jurkat cells labelling IKZF3 (green) with purified ab139408 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.Control: primary antibody (1/100) and secondary antibody ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

Flow cytometry analysis of Ramos cells labelling IKZF3 with purified ab139408 at 1/90 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

Product References

TNF-alpha blockade induces IL-10 expression in human CD4+ T cells. - TNF-alpha blockade induces IL-10 expression in human CD4+ T cells.

Evans HG, Roostalu U, Walter GJ, Gullick NJ, Frederiksen KS, Roberts CA, Sumner J, Baeten DL, Gerwien JG, Cope AP, Geissmann F, Kirkham BW, Taams LS. Nat Commun. 2014;5:3199.